THz Bragg structures fabricated with additive manufacturing.

The advancement of THz science and technology is desirable to facilitate the application of THz technologies in many sectors. Specialized THz photonic elements for these applications require desirable absorption and refractive characteristics in the THz regime. THz photonic elements can be created with additive manufacturing, and specifically 3D printing, forgoing the need for complex fabrication procedures and methodologies. Such THz photonic elements include periodic Bragg structures, which are capable of filtering specific THz frequencies. The authors present a THz Bragg structure fabricated with 3D printing via fused filament fabrication. The THz Bragg structure is made from high-impact polystyrene filament material, which is characterized in this paper with THz time-domain spectroscopy. The geometry and theoretical operation of the THz Bragg structure is investigated with finite-difference time-domain electromagnetic simulations. The THz Bragg structure is evaluated using a THz experimental test bed. There is agreement between the theoretical and the experimental filtering placement within the frequency domain for the THz Bragg structure. The capability of tunable frequency filtering of the presented THz Bragg structure, fabricated with 3D printing, is established and facilitates future advancements in applications of THz science and technology.

A velocity program using the Kanade-Lucas-Tomasi feature-tracking algorithm with demonstration for pressure and electroosmosis conditions.

Investigating microfluidic flow profiles is of interest in the microfluidics field for the determination of various characteristics of a lab-on-a-chip system. Microparticle tracking velocimetry uses computational methods upon recording video footage of microfluidic flow to ultimately visualize motion within a microfluidic system across all frames of a video. Current methods are computationally expensive or require extensive instrumentation. A computational method suited to microparticle tracking applications is the robust Kanade-Lucas-Tomasi (KLT) feature-tracking algorithm. This work explores a microparticle tracking velocimetry program using the KLT feature-tracking algorithm. The developed program is demonstrated using pressure-driven and EOF and compared with the respective mathematical fluid flow models. An electrostatics analysis of EOF conditions is performed in the development of the mathematical using a Poisson's Equation solver. This analysis is used to quantify the zeta potential of the electroosmotic system. Overall, the KLT feature-tracking algorithm presented in this work proved to be highly reliable and computationally efficient for investigations of pressure-driven and EOF in a microfluidic system.

A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin.

Antibiotics are classes of antimicrobial substances that are administered widely in the field of veterinary science to promote animal health and feed efficiency. Cattle-administered antibiotics hold a risk of passing active residues to milk, during the milking process. This becomes a public health concern as these residues can cause severe allergic reactions to sensitive groups and considerable economic losses to the farmer. Hence, to ensure that the produced milk is safe to consume and adheres to permissible limits, an on-farm quick and reliable test is essential. This study illustrates the design and development of a microfluidic paper biosensor as a proof-of-concept detection system for gentamicin in milk. Localized surface plasmon resonance (LSPR) properties of gold nanoparticles have been explored to provide the user a visual feedback on the test, which was also corroborated by RGB analysis performed using Image J. The assay involves the use of a short stretch of single stranded DNA, called aptamer, which is very specific to the gentamicin present in the milk sample. The camera-based LOD for the fabricated paper device for milk samples spiked with gentamicin was calculated to be 300 nM, with a reaction time of 2 min.

Comparison of nonlinear filtering techniques for photonic systems with blackbody radiation.

This work explores a theoretical solution for noise reduction in photonic systems using blackbody radiators. Traditionally, signal noise can be reduced by increasing the integration time during signal acquisition. However, increasing the integration time during signal acquisition will reduce the acquisition speed of the signal. By developing and applying a filter using a model based on the theoretical equations for blackbody radiation, the noise of the signal can be reduced without increasing integration time. In this work, three filters, extended Kalman filter, unscented Kalman filter (UKF), and extended sliding innovation filter (ESIF), are compared for blackbody photonic systems. The filters are tested on a simulated signal from five scenarios, each simulating different experimental conditions. In particular, the nonlinear filters, UKF and ESIF, showed a significant reduction of noise from the simulated signal in each scenario. The results show great promise for photonic systems using blackbody radiators that require post-process for noise reduction.

Voltage control for microchip capillary electrophoresis analyses.

This paper presents an inexpensive and easy-to-implement voltage sequencer instrument for use in microchip capillary electrophoresis (MCE) actuation. The voltage sequencer instrument takes a 0-5 V input signal from a microcontroller and produces a reciprocally proportional voltage signal with the capability to achieve the voltages required for MCE actuation. The unit developed in this work features four independent voltage channels, measures 105 × 143 × 45 mm (width × length × height), and the cost to assemble is under 60 USD. The system is controlled by a peripheral interface controller and commands are given via universal serial bus connection to a personal computer running a command line graphical user interface. The performance of the voltage sequencer is demonstrated by its integration with a fluorescence spectroscopy MCE sensor using pinched sample injection and electrophoretic separation to detect ciprofloxacin in samples of milk. This application is chosen as it is particularly important for the dairy industry, where fines and health concerns are associated with the shipping of antibiotic-contaminated milk. The voltage sequencer instrument presented represents an effective low-cost instrumentation method for conducting MCE, thereby making these experiments accessible and affordable for use in industries such as the dairy industry.

Microchip capillary electrophoresis dairy device using fluorescence spectroscopy for detection of ciprofloxacin in milk samples.

Detecting antibiotics in the milk supply chain is crucial to protect humans from allergic reactions, as well as preventing the build-up of antibiotic resistance. The dairy industry has controls in place at processing facilities, but controls on dairy farms are limited to manual devices. Errors in the use of these manual devices can result in severe financial harm to the farms. This illustrates an urgent need for automated methods of detecting antibiotics on a dairy farm, to prevent the shipment of milk containing antibiotics. This work introduces the microchip capillary electrophoresis dairy device, a low-cost system that utilizes microchip capillary electrophoresis as well as fluorescence spectroscopy for the detection of ciprofloxacin contained in milk. The microchip capillary electrophoresis dairy device is operated under antibiotic-absent conditions, with ciprofloxacin not present in a milk sample, and antibiotic-present conditions, with ciprofloxacin present in a milk sample. The response curve for the microchip capillary electrophoresis dairy device is found through experimental operation with varied concentrations of ciprofloxacin. The sensitivity and limit of detection are quantified for the microchip capillary electrophoresis dairy device.

Scalable optical annealing of microfluidic droplets via whispering gallery mode geometry and infrared illumination.

This work presents a solution to limitations on scalability in traditional on-chip optofluidic polymerase chain reaction (PCR) methods that are based on infrared annealing and droplet-based microfluidics. The scalability in these PCR optofluidic methods is limited by the optical penetration depth of light in a fluid droplet. Traditionally, such an implementation has minimal absorption when the droplet diameter is scaled well below the optical penetration depth due to the small interaction length. In the presented whispering gallery mode (WGM) optofluidic method, a WGM wave is created through total internal reflection, where light is trapped within a droplet. The effect of the trapped light can extend the interaction length beyond the penetration depth, even for small diameter droplets. Thus, this WGM wave permits the use of droplets with diameters scaled below the penetration depth of the light. A theoretical analysis of traditional optical annealing and of the WGM optofluidic method is conducted using finite-difference time-domain analyses. The WGM wave optofluidic method is also demonstrated experimentally, providing higher annealing temperatures than traditional optical annealing. It is envisioned that the presented work will allow for scalable PCR devices implemented on-chip.

Characterisation of graphene electrodes for microsystems and microfluidic devices.

Fabrication of microsystems is traditionally achieved with photolithography. However, this fabrication technique can be expensive and non-ideal for integration with microfluidic systems. As such, graphene fabrication is explored as an alternative. This graphene fabrication can be achieved with graphite oxide undergoing optical exposure, using optical disc drives, to impose specified patterns and convert to graphene. This work characterises such a graphene fabrication, and provides fabrication, electrical, microfluidic, and scanning electron microscopy (SEM) characterisations. In the fabrication characterisation, a comparison is performed between traditional photolithography fabrication and the new graphene fabrication. (Graphene fabrication details are also provided.) Here, the minimum achievable feature size is identified and graphene fabrication is found to compare favourably with traditional photolithography fabrication. In the electrical characterisation, the resistivity of graphene is measured as a function of fabrication dose in the optical disc drive and saturation effects are noted. In the microfluidic characterisation, the wetting properties of graphene are shown through an investigation of the contact angle of a microdroplet positioned on a surface that is treated with varying fabrication dose. In the SEM characterisation, the observed effects in the previous characterisations are attributed to chemical or physical effects through measurement of SEM energy dispersive X-ray spectra and SEM images, respectively. Overall, graphene fabrication is revealed to be a viable option for development of microsystems and microfluidics.

Optofluidic lenses with horizontal-to-vertical aspect ratios in the subunit regime.

Elliptical optofluidic lenses can provide tunable optical parameters in different optical planes. This tunability is achieved through modifications to the aspect ratio (AR). We present an optofluidic lens with a subunit AR. In our experimental analysis, we alter the shape of the microdroplet and observe a 10% reduction in the AR on applying a high voltage across the microdroplet. In our theoretical analysis, we observe improved tunability of focal length, longitudinal spherical aberration, and beam cone angle in the subunit AR regime compared to the superunit AR regime. We ultimately test and characterize the optofluidic lens in an imaging application.

Photoconductive terahertz generation from textured semiconductor materials.

Photoconductive (PC) terahertz (THz) emitters are often limited by ohmic loss and Joule heating-as these effects can lead to thermal runaway and premature device breakdown. To address this, the proposed work introduces PC THz emitters based on textured InP materials. The enhanced surface recombination and decreased charge-carrier lifetimes of the textured InP materials reduce residual photocurrents, following the picosecond THz waveform generation, and this diminishes Joule heating in the emitters. A non-textured InP material is used as a baseline for studies of fine- and coarse-textured InP materials. Ultrafast pump-probe and THz setups are used to measure the charge-carrier lifetimes and THz response/photocurrent consumption of the respective materials and emitters. It is found that similar temporal and spectral characteristics can be achieved with the THz emitters, but the level of photocurrent consumption (yielding Joule heating) is greatly reduced in the textured materials.

Ultrafast all-optical technologies for bidirectional optical wireless communications.

In this Letter, a spherical retro-modulator architecture is introduced for operation as a bidirectional transceiver in passive optical wireless communication links. The architecture uses spherical retroreflection to enable retroreflection with broad directionality (2π steradians), and it uses all-optical beam interaction to enable modulation on ultrafast timescales (120 fs duration). The spherical retro-modulator is investigated from a theoretical standpoint and is fabricated for testing with three glasses, N-BK7, N-LASF9, and S-LAH79. It is found that the S-LAH79 structure provides the optimal refraction and nonlinearity for the desired retroreflection and modulation capabilities.

Nanophotonic implementation of optoelectrowetting for microdroplet actuation.

The development and ultimate operation of a nanocomposite high-aspect-ratio photoinjection (HARP) device is presented in this work. The device makes use of a nanocomposite material as the optically active layer and the device achieves a large optical penetration depth with a high aspect ratio which provides a strong actuation force far away from the point of photoinjection. The nanocomposite material can be continuously illuminated and the position of the microdroplets can, therefore, be controlled to diffraction limited resolution. The nanocomposite HARP device shows great potential for future on-chip applications.

Retroreflective imaging system for optical labeling and detection of microorganisms.

A retroreflective imaging system for imaging microscopic targets over macroscopic sampling areas is introduced. Detection of microorganism-bound retroreflector (RR) targets across millimeter-scale samples is implemented according to retroreflection directionality, collimation, and contrast design characteristics. Retroreflection directionality is considered for corner-cube (CC) and spherical geometries. Spherical-RRs improve directionality and reliability. Retroreflection collimation is considered for spherical-RRs. Retroreflective images for micro-CC-RRs and micro-spherical-RRs with varying refractive indices show optimal results for high refractive index BaTiO3 micro-spherical-RRs. A differential imaging technique improves retroreflection contrast by 35 dB. High refractive index micro-spherical-RRs and differential imaging, together, can detect microscopic RR targets across macroscopic areas.

Ultrafast transient responses of optical wireless communication detectors.

In this work, fundamental ultrafast transient responses are studied for optical wireless communication (OWC) detectors. It is shown that material impulse responses, associated with transient photoconductivity, and geometrical input responses, associated with transient optical power, must be considered in tandem when OWC photodetection is pursued with broad spectral and directional characteristics. An OWC detector, composed of GaAs photoconductive gaps in a corner-cube geometry, is fabricated and analyzed. The GaAs material response times are investigated experimentally and found to range from approximately 3 ps to 200 fs for 390 nm (violet) to 780 nm (red) photoexcitation. The geometrical response times are investigated theoretically and found to range from approximately 2 to 20 ps for device dimensions from 1 to 10 mm. The overall response times manifest themselves in two distinct dimensional regimes, with differing levels of wavelength and dimension dependence. The relevance of these findings is discussed for future ultrafast OWC detectors.

On-chip digital microfluidic architectures for enhanced actuation and sensing.

An on-chip system is presented with integrated architectures for digital microfluidic actuation and sensing. Localized actuation is brought about by a digital microfluidic multiplexer layout that overcomes the challenges of multi-microdrop interference, and complete two-dimensional motion is shown for microdrops on a 14 × 14 grid with minimized complexity by way of 14+14 inputs. At the same time, microdrop sensing is demonstrated in a folded-cavity design for enhanced optical intensity probing of internal fluid refractive indices. The heightened intensities from this on-chip refractometer are shown to have a linear response to the underlying fluid refractive index. An electro-dispensing technique is used to fabricate the folded-cavity optical architecture in a format that is tuned for the desired refractive index range and sensitivity. The overall lab-on-a-chip system is successful in integrating localized microdrop actuation and sensing.